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BMS 535 Advanced Topics in Recombinant DNA Technology: Gene Transfer, Expression, and Detection (Laboratory–Lecture course)

Prerequisite: BMS 534 or permission of the instructor. (Alternate summers/3 credits/lab fee)

A study of the theory and techniques for the introduction, expression and detection of macromolecules in eukaryotic cells. The topics to be covered include the introduction of recombinant genes in eukaryotic hosts by transfection, lipofection and microinjection. Methodologies to produce transgenic animals will be discussed. Analysis of eukaryotic gene expression and detection of activity, northern and western analysis and fluorescent in situ hybridization to chromosomes.